Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEAntimicrobialagent-5Cat.No.:HY-151399分?式:C??H??N??分?量:656.83作?靶點(diǎn):Bacterial作?通路:Anti-infection儲存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性Antimicrobialagent-5?種強(qiáng)效抗劑，對?蘭?陽性/性都具有良好的細(xì)胞選擇性。Antimicrobialagent-5能夠阻斷LPS和CD14/TLR4受體間的相互作?，表現(xiàn)出抗炎活性并緩解LPS誘導(dǎo)的炎癥。體外研究Antimicrobialagent-5(compound9)(0.5-32μg/mL,16h;1-128μg/mL;24h)showspotentbiofilminhibitory(IC50=2μg/mL)anderadicatingactivities(IC50=16μg/mL)againstmultidrug-resistantPseudomonasaeruginosa(MDRPA)[1].Antimicrobialagent-5(5μgmL,20μg/mL;18h)inhibitsboththereleaseandexpressionofnitricoxide(NO)andtumornecrosisfactor-α(TNF-α)fromLPS-stimulated(1μg/mL)RAW264.7cells[1].Antimicrobialagent-5exhibitsproteolyticresistanceandsalt/serumstability[1].Antimicrobialagent-5(0.5-256μg/mL;2h)exhibitsnegligiblesideeffectsagainstsheepredbloodcells(sRBCs)withhemolyticactivity(theminimumhemolyticconcentration,MHC)of>256μg/mL[1].CellViabilityAssay[1]CellLine:RAW264.7cellsConcentration:5μgmL,20μg/mLIncubationTime:18hoursResult:DecreasedTNF-αreleaseat20μg/mL,withinhibitionrateof72.44%.ResultsreductionintheLPS-stimulatedproductionofNO,withinhibitionrateof31.51%.CellViabilityAssay[1]1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECellLine:E.coli[KCTC1682],P.aeruginosa[KCTC1637],S.epidermidis[KCTC1917],S.aureus[KCTC1621]Concentration:1-128μg/mLIncubationTime:24hoursResult:InhibitedGram-negativebacteriaandGram-positivebacteriawithIC50of6.1μM(E.coli[KCTC1682],P.aeruginosa[KCTC1637],S.epidermidis[KCTC1917],S.aureus[KCTC1621]),respectively.REFERENCES[1].DineshKumarS,etal.Cationic,amphipathicsmallmoleculesbasedonatriazine-piperazine-triazinescaffoldasanewclassofantimicrobialagents.EurJMedChem.2022Sep8;243:114747.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel: