1、Product Data SheetMangiferinCat. No.: HY-N0290CAS No.: 4773-96-0分式: CHO分量: 422.34作靶點: NF-B; Keap1-Nrf2; Apoptosis作通路: NF-B; Apoptosis儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 50 mg/mL (118.39 mM; Need ultrasonic)SolventMass1 mg 5 mg 10 mgConcentration制備儲備

2、液1 mM 2.3678 mL 11.8388 mL 23.6776 mL5 mM 0.4736 mL 2.3678 mL 4.7355 mL10 mM 0.2368 mL 1.1839 mL 2.3678 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲存時，請在 6 個內(nèi)使，-20C 儲存時，請在 1 個內(nèi)使。體內(nèi)實驗請根據(jù)您的實驗動物和給藥式選擇適當(dāng)?shù)娜芙獍浮Ｒ韵氯芙獍付颊埾劝凑?In Vitro 式配制澄清的儲備液，再依次添加助溶

3、劑：為保證實驗結(jié)果的可靠性，澄 的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.92 mM); Clear solution此案可獲得 2.5 mg/mL (5.92 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 4

4、00 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (5.92 mM); Clear solution此案可獲得 2.5 mg/mL (5.92 mM，飽和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄均勻。DMSO 儲備液加到 900 L 20% 的 SBE-CD 理鹽溶液中，混合3

5、. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (5.92 mM); Clear solution此案可獲得 2.5 mg/mL (5.92 mM，飽和度未知) 的澄 溶液，此案不適于實驗周 期在半個以上的實驗。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Mangiferin種 Nrf2 激活劑。Mangiferin 抑制 NF-B 亞基p65 和 p50 的核移位。IC & Target p65 p50 Nrf

6、2體外研究 Mangiferin is glucosylxanthone extracted from plants of the Anacardiaceae and Gentianaceae families. Mangiferin (50M) significantly increases Nrf2 protein accumulation in HL-60 cells, particularly in the nucleus. Mangiferin alsoenhances the binding of Nrf2 to an antioxidant response element (A

7、RE), significantly up-regulates NAD(P)H:quinoneoxidoreductase 1 (NQO1) expression and reduces intracellular ROS in HL60 cells. Mangiferin alone dose-dependentlyinhibits the proliferation of HL-60 cells. In mononuclear cells (MNCs), Mangiferin significantly relieves oxidative stress,but attenuates et

8、oposide-induced cytotoxicity1. Mangiferin is a natural phytopolyphenol that exhibits variouspharmacological properties. Mangiferin is present in several plant species such as Mangifera indica, Iris unguicularis,and Anemarrhena asphodeloides. Mangiferin downregulates TNF-induced MMP-9 mRNA and protei

9、n expressionby suppressing NF-B activity, consequently suppressing the invasion of LNCaP cells.To assess whether Mangiferininfluences the viability of LNCaP cells, MTT assay is performed 24 h after treatment with different concentrations ofMangiferin in the presence or absence of TNF- in serum and s

10、erum-free conditions. There are no cytotoxic evidentin LNCaP cells treated with up to 400 M of Mangiferin alone under both serum and serum-free conditions.Additionally, in the presence of TNF- (20 ng/mL), Mangiferin does not affect cell viability. Mangiferin (400 M)treatment significantly decreases

11、the NF-B luciferase activity in TNF-stimulated LNCaP cells. Pretreatment withMangiferin (400 M) for 1 h significantly decreases TNF-induced NF-B activity. The RT-PCR shows that Mangiferin(400 M) significantly suppresses the TNF-induced MMP-9 expression in LNCaP cells2.PROTOCOLCell Assay 2 LNCaP cell

12、s are cultured at 37C in 5% CO2 in RPMI supplemented with 10% FBS and antibiotics. Cell viability isdetermined by an MTT assay. Briefly, LNCaP cells (1105 cells/mL) are plated onto 24 well plates and incubatedovernight in serum and serum-free RPMI media. The cells are treated with the indicated conc

13、entrations of Mangiferin(100, 200, and 400 M) for 1 h and then stimulated with TNF- (20 ng/mL) for 24 h. Then, the cells are incubatedwith a solution of 0.5 mg/mL MTT and incubation for 45 min at 37C and 5% CO2. Supernatant is removed and theformation of formazan is observed by monitoring the signal

14、 at 540 nm using a microplate reader2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303.See more customer validations on HYPERLINK www.MedChemE www.MedChemEPage 2 of 3 www.MedChemEREFERENCE

15、S1. Zhang BP, et al. Mangiferin activates Nrf2-antioxidant response element signaling without reducing the sensitivity to etoposide of human myeloidleukemia cells in vitro. Acta Pharmacol Sin. 2014 Feb;35(2):257-66.2. Dilshara MG, et al. Mangiferin inhibits tumor necrosis factor-induced matrix metalloproteinase-9 expression and cellular invasion by suppressingnuclear factor-B activity. BMB Rep. 2015 Oct;48(10