Hotline:400-820-3792Inhibitors ? ScreeningLibraries ? Proteinswww.MedChemEStigmast-5-en-3-olCat.No.:HY-N12999CASNo.:5779-62-4分子式:C??H??O分子量:414.71作用靶點:Apoptosis;Bcl-2Family;Caspase;MDM-2/p53;PARP;Amylases作用通路:Apoptosis;CellCycle/DNADamage;Epigenetics;MetabolicEnzyme/Protease儲存方式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY生物活性Stigmast-5-en-3-ol誘導(dǎo)癌細(xì)胞凋亡(apoptosis)和抑制增殖通過增加Bax、Caspase-9、p53和PARP裂解的產(chǎn)生，降低Bcl-xl表達(dá)。Stigmast-5-en-3-ol對葡萄糖淀粉酶和α-淀粉酶(α-amylase)表現(xiàn)出強效抑制活性，具有較高的抗氧化活性。Stigmast-5-en-3-ol可用于白血病、乳腺癌、2型糖尿病、肥胖癥等疾病的研究。IC50&TargetCaspase-9BaxBcl-xL體外研究Stigmast-5-en-3-ol(CompoundDGEH21-3-2)(12.5-50μg/mL,24h)showscytotoxiceffectsagainstHL-60andMCF-7cells,withIC50sof37.82and45.17?μg/mLrespectively[1].Stigmast-5-en-3-ol(12.5-50μg/mL,24h)inducesapoptosis,increasesDNAdamage,increasesproductionofBax,Caspase-9,p53andPARPcleavage,anddecreasedBcl-xllevelsinHL-60andMCF-7cells[1].Stigmast-5-en-3-ol(Compound24R)(2-10μg/mL,30min)exhibitspotentinhibitoryactivityagainstglucoamylaseandα-amylasewithIC50sof5.8and7.198μg/mLrespectively,showshighradicalactivity[2].Apoptosis[1]CellLine:HL-60andMCF-7cellsConcentration:12.5,25,50μg/mLIncubationTime:24hResult:Increasedthenumberofapoptoticbodiesanddose-dependentlyincreasedthesub-G1apoptoticcellpopulation,resultinginasub-G1apoptoticcellpopulationof66.30%inHL-1/2 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemE60cellsand60.05%inMCF-7cellsat50μg/ml.IncreasedproductionofBax,Caspase-9,andPARPcleavage,anddecreasedBcl-xllevels.Dose-dependentincreasedp53expressioninMCF-7cells.REFERENCESFernandoIPS,etal.ApoptoticandantiproliferativeeffectsofStigmast-5-en-3-olfromDendronephthyagiganteaonhumanleukemiaHL-60andhumanbreastcancerMCF-7cells.ToxicolInVitro.2018Oct;52:297-305.BrindaBolar,etal.ISOLATIONANDBIOLOGICALEVALUATIONOF(24R)STIGMAST5-EN-3-OLFROMCASSIAFISTULABARK.DinaA.Ghinmy,etal.INVESTIGATIONOFANTI-OBESITYACTIVITYOFETHANOLICEXTRACTOFFOENICULUMVULGARESEEDS,INVIVOANDINSILICOMODELS.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuse