Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEZ-YVAD-FMKCat.No.:HY-P1009CASNo.:210344-97-1分?式:C??H??FN?O?分?量:630.66Sequence:Z-Tyr-Val-Ala-Asp-FMKSequenceShortening:Z-YVAD-FMK作?靶點(diǎn):Caspase作?通路:Apoptosis儲存?式:Powder-80°C2years-20°C1yearInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:125mg/mL(198.21mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.5856mL7.9282mL15.8564mL5mM0.3171mL1.5856mL3.1713mL10mM0.1586mL0.7928mL1.5856mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(3.30mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(3.30mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(3.30mM);ClearsolutionBIOLOGICALACTIVITY?物活性Z-YVAD-FMK?種具有細(xì)胞通透性caspase-1和caspase-4抑制劑，具有抗炎和抗腫瘤活性。IC50&TargetCaspase體外研究Z-YVAD-FMK(100μM;24hours)significantlydownregulatedthegrowthinhibitioninducedbybutyrateinCaco-2cells[1].Z-YVAD-FMK(20μM;pre1hour;24hours)attenuatestheapoptoticinductionofIII-10onbothHepG2andBEL-7402cells,theapoptoticrateof-10onHepG2cellsisreducedbyZ-VAD-FMKfrom19.88%to8.34%,whilethatonBEL-7402cellsisreducedfrom17.56%to11.98%[4].CellViabilityAssay[1]CellLine:Caco-2cellsConcentration:0-100μMIncubationTime:24hoursResult:InhibitedCaco-2cellsgrowth.ApoptosisAnalysis[1]CellLine:BEL-7402andHepG2cellsConcentration:20μMIncubationTime:Pre1hour;24hoursResult:Inducedacaspase-dependentapoptosisincells.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?JHazardMater.2021Jan13;411:125134.?JAllergyClinImmunol.2022Mar26;S0091-6749(22)00382-7.?BiomedPharmacother.2022Jul;151:113098.?JVirol.2022May25;96(10):e0030922.?HumCell.2022Feb25.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].LiH1,etal.Aluminumhydroxideadjuvantsactivatecaspase-1andinduceIL-1betaandIL-18release.JImmunol.2007Apr15;178(8):5271-6.[2].Avivi-GreenC,etal.Differentmoleculareventsaccountforbutyrate-inducedapoptosisintwohumancoloncancercelllines.JNutr.2002Jul;132(7):1812-8.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplica