Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemED-AP5Cat.No.:HY-100714ACASNo.:79055-68-8Synonyms:D-APV;D-2-Amino-5-phosphonovalericacid分?式:C?H??NO?P分?量:197.13作?靶點:iGluR作?通路:MembraneTransporter/IonChannel;NeuronalSignaling儲存?式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗H2O:27.78mg/mL(140.92mM;Needultrasonic)DMSO:<1mg/mL(ultrasonic;warming;heatto60°C)(insolubleorslightlysoluble)Ethanol:<1mg/mL(ultrasonic)(insoluble)MassSolvent1mg5mg10mgConcentration制備儲備液1mM5.0728mL25.3640mL50.7279mL5mM1.0146mL5.0728mL10.1456mL10mM0.5073mL2.5364mL5.0728mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請依序添加每種溶劑：PBSSolubility:100mg/mL(507.28mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性D-AP5(D-APV)?種選擇性和競爭性NMDA受體拮抗劑，Kd為1.4μM。D-AP5抑制NMDA受體的?氨酸結合位點。IC50&TargetNMDA[1]體內研究D-AP5isaNMDAreceptorantagonist.ChronicintraventricularinfusionofD-AP5causesaparalleldose-dependentimpairmentofspatiallearningandlong-termpotentiation(LTP)invivo.IntracerebralconcentrationsofD-AP5failtoinduceanymeasurablesensorimotordisturbancewhenspatiallearningisprevented[2].D-AP5infusionisassociatedwithaprogressivereductioninswimspeedovertrials.D-AP5causessensorimotordisturbancesinthespatialtask,butthesegraduallyworsenedastheanimalsfailtolearn.RatstreatedwithD-AP5showadelay-dependentdeficitinspatialmemoryinthedelayedmatching-to-placeprotocolforthewatermaze[3].PROTOCOLAnimalMaleListerhoodedratsareusedinthisstudy.TheyarehousedindividuallyandhavefreeaccesstofoodAdministration[1]andwateratalltimesexceptfor12hrpriortosurgery.Animalsareimplantedwith30mMD-AP5(N=6)andtestedinthewatermaze[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻?Theranostics.2021Mar4;11(10):4616-4636.?SensActuatorsBChem.2020,129190.?BiomedPharmacother.January2022,112446.?CellDeathDiscov.2020Sep17;6:87.?CNSNeurosciTher.2022Nov15.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].DavisS,etal.TheNMDAreceptorantagonistD-2-amino-5-phosphonopentanoate(D-AP5)impairsspatiallearningandLTPinvivoatintracerebralconcentrationscomparabletothosethatblockLTPinvitro.JNeurosci.1992Jan;12(1):21-34.[2].MorrisRG,etal.N-methyl-d-aspartatereceptors,learningandmemory:chronicintraventricularinfusionoftheNMDAreceptorantagonistd-AP5interactsdirectlywiththeneuralmechanismsofspatiallearning.EurJNeurosci.2013Mar;37(5):700-17.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE[3].RHEvans,etal.Theeffectsofaseriesofomega-phosphonicalpha-carboxylicaminoacidsonelectricallyevokedandexcitantaminoacid-inducedresponsesinisolatedspinalcordpreparations.BrJPharmacol.1982Jan;75(1):65-75.McePdfHeightCaution:Producthasnotbeenf