1、Product Data SheetMizoribineCat. No.: HY-17470CAS No.: 50924-49-7分式: CHNO分量: 259.22作靶點: HCV; SARS-CoV作通路: Anti-infection儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 35 mg/mL (135.02 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcent

2、ration制備儲備液1 mM 3.8577 mL 19.2886 mL 38.5773 mL5 mM 0.7715 mL 3.8577 mL 7.7155 mL10 mM 0.3858 mL 1.9289 mL 3.8577 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲存時，請在 6 個內(nèi)使，-20C 儲存時，請在 1 個內(nèi)使。體內(nèi)實驗請根據(jù)您的實驗動物和給藥式選擇適當?shù)娜芙獍?。以下溶解案都請先按?In Vitro 式配制澄清的儲

3、備液，再依次添加助溶劑：為保證實驗結(jié)果的可靠性，澄 的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (9.64 mM); Clear solution此案可獲得 2.5 mg/mL (9.64 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DM

4、SO 儲備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (9.64 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 2.5 mg/mL (9.64 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 900 L 20% 的 SBE-CD 理

5、鹽溶液中，混合均勻。3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (9.64 mM); Clear solution此案可獲得 2.5 mg/mL (9.64 mM，飽和度未知) 的澄 溶液，此案不適于實驗周 期在半個以上的實驗。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Mizoribine (NSC 289637) 抑制 HCV RNA 復(fù)制，具有抗 HCV 活性，IC50 約為 100 M。Mizori

6、bine種免疫抑制劑1。Mizoribine 抑制 IMPDH。Mizoribine 抑制 SARS-CoV 復(fù)制，對 SARS-CoV Frankfurt-1 和 SARS-CoV HKU39849 的IC50 分別為 3.5 g/mL 和 16 g/mL2。IC & Target HCV1; IMPDH; SARS-CoV2體外研究 Mizoribine, isolated from culture medium of the mold Eupenicillium brefeldianum M-2166, acts as animmunosuppressant which exerts i

7、ts effects without severe side effects1.Mizoribine is an inosine-5-monophosphate dehydrogenase (IMPDH) inhibitorused for renal transplantation,autoimmune diseases and steroid-resistant nephrotic syndrome 2.體內(nèi)研究 Mizoribine suppresses collagen-induced arthritis. Mizoribine (10, 20 and 50 mg/kg; Admini

8、stered orally 5 days a weekfor 12 weeks) reduces the arthritis score in a dose-dependent fashion, showing significant suppression, even at a doseof 10 mg/kg3.Animal Model: Male DBA/1 J mice with collagen-induced arthritis3Dosage: 10, 20 and 50 mg/kgAdministration: Administered orally 5 days a week f

9、or 12 weeksResult: Showed dose-dependency of the suppressive effect.戶使本產(chǎn)品發(fā)表的科研獻 Molecules. 2019 May 3;24(9). pii: E1723.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Naka K, et al. Mizoribine inhibits hepatitis C virus RNA replication: effect of combination with in

10、terferon-alpha. Biochem Biophys Res Commun. 2005 May13;330(3):871-9.2. Saijo M, et al. Inhibitory effect of Mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus.Antiviral Res. 2005 Jun;66(2-3):159-63.Page 2 of 3 www.MedChemE3. Kamada H, et al. Effect of Mizoribine on collagen-induced arthritis in mice. Jpn J Pharmacol. 1996 Feb;70(2):169-75.McePdfHeightCaution: Product has not been fully validated for medic