紀(jì)念安徽針灸經(jīng)絡(luò)研究50周年穴位肥大細(xì)胞及其顆粒物

在針刺鎮(zhèn)痛中的作用

針灸經(jīng)絡(luò)研究回顧與展望國際學(xué)術(shù)研討會2010.10.16Outline穴位肥大細(xì)胞在針刺鎮(zhèn)痛效應(yīng)中的作用穴位膠原纖維在針刺鎮(zhèn)痛效應(yīng)中的作用穴位注射組胺的鎮(zhèn)痛效應(yīng)手針和電針穴位信號的啟動機(jī)制MastcellsinacupointsandsidewardComparisonofNumberofMCsinZhushanliandnon-acupointsofRatGroupNameMCsNumberSkinMuscleAcupoint（n＝6）34.2±2.512.4±1.4Non-acupoint（n＝6）22.1±1.7*8.5±1.0*THEJOURNALOFSCIENCE&HEALING,2008，4（3），170-1771.Therelationshipbetween

mastcellfunctionandacupunctureeffectAnimalmodel：AA(acutearthritis)RatCompleteFreunds’adjuvantAcupointselected：Zushanli(ST36)

DST

36C1.Therelationshipbetween

mastcellfunctionandacupunctureeffectTreateffect：Easepain，Painthreshold1.Therelationshipbetween

mastcellfunctionandacupunctureeffectThedegranulationofmastcellsSDRatsTailFlickModelAnatomyPositionofZusanliAcupunctureInjectiondrugsforpreventdegranulationofMCABDEST

36CPainthresholdofratwhenshieldmastcelldegranulation1.Therelationshipbetween

mastcellfunctionandacupunctureeffectIDGroupName組別Average±SEM(%)AControl正常對照組–1.9±0.1B1AcupunctureatST36穴位針刺組11.6±0.6*B2AcupunctureatNearby-ST36旁開針刺組8.9±0.3*△△C1InjectionofDSCGatST36色甘酸鈉注射組1.7±0.1*△C2InjectionofNaClatST36生理鹽水注射組1.4±0.1*△D1AcupunctureafterDSCGatST36色甘酸鈉注射＋針刺組6.2±0.4*△△D2AcupunctureafterNaClatST36生理鹽水注射＋針刺組8.9±0.4*▲D3AcupunctureoppositesideafterDSCG色甘酸鈉注射＋對側(cè)針刺組9.3±0.1*▲▲各組別大鼠痛閾變化率峰值比較（20分鐘時）(n=6)Note:*P<0.05vs.GroupA;△P<0.01,

△△P<0.05vs.GroupB1;▲P<0.01,

▲▲P<0.05vs.GroupD11.Therelationshipbetween

mastcellfunctionandacupunctureeffectGroupName組別MCDegranulationRatio

(Average±SEM)(％)SkinMuscleControl正常對照組29.2±1.226.3±1.8Acupuncture穴位針刺組48.9±3.9*32.2±3.1**AcupunctureafterDSCG色甘酸鈉注射＋針刺組33.7±2.8△28.5±2.3AcupunctureafterNaCl生理鹽水注射＋針刺組50.4±2.5*29.7±2.3穴區(qū)組織肥大細(xì)胞脫顆粒率統(tǒng)計(jì)

(n=6)Note:*P<0.01,**P<0.05vs.ControlGroup;△P<0.01vs.AcupunctureGroup.ResultsfromExperimentStainingofMastCellsinAcupointonRatsMuscleSkinBeforeAcupunctureAfterAcupunctureDegranulationofcellsAfterSDCGStabilizedTHEJOURNALOFSCIENCE&HEALING,2008，4（3），170-177WhatisthekeyfactortodegranulationTheacupunctureeffectispositiverelationshiptothedegranulationofmastcellatacupoint.Whatiskeyfactorconducethedegranulationofmastcellduringacupuncturemanipulation?Ourfocusisonthecollagenatacupoint.2025/8/1713ToobservethefunctionofcollagenfibersinMAanalgesia.7

Animalgroupssetting:normalgroup(N):Normalhealthyanimalcontrolgroup(M):AnimalwithAAinjectionofcollagenaseatZusanli(ST36)group(C)manualacupuncturegroupwithrotationmanipulation(R)orlift-thrustmanipulation(L),rotationgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CR),lift-thrustgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CL),lift-thrustgrouppretreatedwithcollagenasetodestroyDubi(ST35)collagenfibers(CDL)(R)(L)2025/8/1714AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：較正常組痛閾：*P<0.01；較處理前痛閾：

P<0.05；較捻轉(zhuǎn)組(R)痛閾變化率：▽P<0.05；較捻轉(zhuǎn)組(L)痛閾變化率：▼P<0.05；較正常組或模型組:

×P<0.05；較捻轉(zhuǎn)組或提插組:

△P<0.05。2025/8/1715AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：較正常組痛閾：*P<0.01；較處理前痛閾：

P<0.05；較捻轉(zhuǎn)組(R)痛閾變化率：▽P<0.05；較捻轉(zhuǎn)組(L)痛閾變化率：▼P<0.05；較正常組或模型組:

×P<0.05；較捻轉(zhuǎn)組或提插組:

△P<0.05。2025/8/1716AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：較正常組痛閾：*P<0.01；較處理前痛閾：

P<0.05；較捻轉(zhuǎn)組(R)痛閾變化率：▽P<0.05；較捻轉(zhuǎn)組(L)痛閾變化率：▼P<0.05；較正常組或模型組:

×P<0.05；較捻轉(zhuǎn)組或提插組:

△P<0.05。collagenfibersofskinandmuscleMastcellsinskinGroupNormalGroupInjectControlcollagenfibersofskinandmuscleMastcellsinskinConnectiveTissueResearch,2008，50（2）:110-120Acupunc.whirlingAcupunc.liftandthrustcollagenfibersofskinandmuscleMastcellsinskincollagenfibersofskinandmuscleMastcellsinskinConnectiveTissueResearch,2008，50（2）:110-120

Mastcellsdegranulationbeforeandaftercollagendestroyed

CollagendestroyedCollagendestroyedConnectiveTissueResearch,2008，50（2）:110-120collagenfibersandmastcellsinnormalacupointcollagenfibersenlacementandmastcellsdegranulationwhilewhirlingcollagenfiberstightenedandmastcellsdegranulatedwhileliftingandthrustingcollagenfibersweredestroyedandmastcellshadnodegranulationConnectiveTissueResearch,2008，50（2）:110-1202025/8/1721Themastcellsinacupointmaytranslatethemechanicalsignalintothebiologicalinformationofneuralsystem.Thecollagenandmastcellsdegranulationareveryimportanttothemanualacupunctureanalgesiaeffect.Isthisthesameaselectricacupuncture?2025/8/1722組別n痛閾(s)痛閾變化率

(%)肥大細(xì)胞脫顆粒率(%)正常組89.19±0.65-10.6模型組85.61±0.48*-14.1手針組87.52±0.65△38.575.4△電針組88.67±1.06△45.973.3△膠原酶+手針組85.39±0.57×3.210.4××膠原酶+電針組88.30±1.2347.619.0▲色甘酸鈉+手針組86.00±0.48××5.825.1××色甘酸鈉+電針組87.34±0.70▲27.529.5▲手針及電針治療前后AA大鼠痛閾和痛閾改變率以及穴區(qū)肥大細(xì)胞脫顆粒率的變化注：與正常組相比較：*P<0.05；與模型組相比較：△P<0.05；與手針組相比較：××P<0.05；×P<0.01；與電針組相比較：▲P<0.05。3.穴位皮下注射組胺的鎮(zhèn)痛療效組別n痛閾(s)基礎(chǔ)痛閾造模后痛閾治療后痛閾空白對照組129.04±0.209.38±0.199.27±0.17模型組128.90±0.406.48±0.28﹡6.58±0.35△生理鹽水對照組129.20±0.286.09±0.30﹡6.61±0.31△手針組129.52±0.186.58±0.17﹡8.77±0.26＊組胺注射組129.21±0.206.27±0.22﹡8.50±0.28＊☆各組痛閾測量比較3.穴位皮下注射組胺的鎮(zhèn)痛療效組別n痛閾(s)基礎(chǔ)痛閾造模后痛閾治療后痛閾空白對照組129.04±0.209.38±0.199.27±0.17模型組128.90±0.406.48±0.28﹡6.58±0.35△生理鹽水對照組129.20±0.286.09±0.30﹡6.61±0.31△手針組129.52±0.186.58±0.17﹡8.77±0.26＊組胺注射組129.21±0.206.27±0.22﹡8.50±0.28＊☆各組痛閾測量比較針刺研究，2010，35（2）：99-1033.穴位皮下注射組胺的鎮(zhèn)痛療效組別n痛閾(s)基礎(chǔ)痛閾造模后痛閾治療后痛閾空白對照組129.04±0.209.38±0.199.27±0.17模型組128.90±0.406.48±0.28﹡6.58±0.35△生理鹽水對照組129.20±0.286.09±0.30﹡6.61±0.31△手針組129.52±0.186.58±0.17﹡8.77±0.26＊組胺注射組129.21±0.206.27±0.22﹡8.50±0.28＊☆各組痛閾測量比較針刺研究，2010，35（2）：99-1033.組胺H1受體在傳遞針刺信號中的作用

各組足三里穴位處脫顆粒比較組別N脫顆粒率空白組1233.59±0.72模型組1239.71±2.09﹡生理鹽水穴位注射組1236.67±2.36手針針刺組1257.61±1.42＊組胺穴位注射組1257.03±2.95＊◆色甘酸鈉預(yù)處理胺注射組1325.40±1.80▼色甘酸鈉預(yù)處理針刺組1236.03±2.28△組胺H1拮抗劑預(yù)處理針刺組1251.54±2.32□組胺H1拮抗劑組胺注射組1237.13