Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemECalmidazoliumchlorideCat.No.:HY-103319CASNo.:57265-65-3Synonyms:R24571分?式:C??H??Cl?N?O分?量:687.7作?靶點(diǎn):CaMK;Autophagy作?通路:NeuronalSignaling;Autophagy儲存?式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(145.41mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.4541mL7.2706mL14.5412mL5mM0.2908mL1.4541mL2.9082mL10mM0.1454mL0.7271mL1.4541mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(3.02mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(3.02mM);ClearsolutionBIOLOGICALACTIVITY?物活性Calmidazoliumchloride(R24571)?種鈣調(diào)蛋?拮抗劑，拮抗CaM依賴性磷酸?酯酶和鈣調(diào)蛋?誘導(dǎo)的紅細(xì)胞Ca2+轉(zhuǎn)運(yùn)ATP酶活化，IC50分別為0.15和0.35μM[1]。也?于抗癌研究[2]。它結(jié)合CaMK的Kd值為3nM[3]。IC50&TargetKd:3nM(Calmodulin)[3]體外研究Calmidazoliumchlorideiswidelyusedasacalmodulin(CaM)antagonist,butisalsoknowntoinduceapoptosisincertaincancercelllines.Calmidazoliumchloride(3,5,7,10μM,30minutes-24hours)inhibitsgrowthofmouseF9ECCs[2].CellViabilityAssay[2]CellLine:MouseF9ECCsConcentration:3,5,7,10μMIncubationTime:30minutes-24hoursResult:TheIC50sofCalmidazoliumchloridetreatedF9ECCsandE14ESCsare8.18μM,and12.69μM[2].REFERENCES[1].GietzenK,etal.Comparisonofthecalmodulinantagonistscompound48/80andcalmidazolium.BiochemJ.1983Dec15;216(3):611-6.[2].LeeJ,etal.Calmidazoliumchlorideinhibitsgrowthofmurineembryonalcarcinomacells,amodelofcancerstem-likecells.ToxicolInVitro.2016Sep;35:86-92.[3].BuduA,etal.CalmidazoliumevokeshighcalciumfluctuationsinPlasmodiumfalciparum.CellSignal.2016Mar;28(3):125-135.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearch