交通大學(xué)生物化學(xué)試卷（2003校

系

專 學(xué) 日 2003 PAPER(1)（閉卷部分(ShanghaiJiaoTongUniversity,1sttermofUniv.yearSubject: Name Class

Score 一Explaintheterms:（2.5x10=LDLpyruvatedehydrogenaseglucokinase(inmalate-aspartatesubstrate“macroureaUDP-2003 PAPER2)（開卷(ShanghaiJiaoTongUniversity,1sttermofUniv.yearSubject: Name Class

Score 二Fillingtheblanks2.5x12=Manyimportantcoenzymes,suchas,and,arethederivativesofADP;dNDParesynthesizedfromanddTMPisformedby ofdUMP;allthesefactsreflectsthatcamebefore inevolution.TPPisthederivativeofvitamin,itservesastheprostheticgroupofthefollowingenzymes: , Thereisalargedifferencebetweentheyieldfromthecompleteoxidationoftriacylglycerolsandofcarbohydrates(andprotein),thereasonis Thethreepairsofenzymesthatcatalyzethethreeresemblestepsinβ-oxidationofFAandcitricacidcycleare , catalyzedby and , and Inthesynthesisofphosphoglycerides磷酸甘油酯andofglycogen,theactivatedintermediatesare ,bothofthemareformedfroma substrateanda ,andthenreactwitha Thecommittedstepincholesterolbiosynthesisisthesynthesis from whichiscatalyzedby Thecarrieroftheactivatedone-carbonunits whichofthree

,

Besides, isthemajordonorofmethylgroup.Thefully

Diseaseandinheriteddefectsofenzymeorfunctionalprotein,e.g.PKU—hydroxylase;drug-inducedhemolyticanemia溶血性貧血— hypercholesterolemia高膽固醇血癥— 性貧血— ;maplesyrup槭糖漿urinedisease ;Leber’sopticneuropathy視神經(jīng)癥 AcetylCoAcarboxylasefromE.coliconsistsofthreesubunits , and Therearetwotypesofcovalentmodification: intheregulationofenzymaticactivity,thetypicalexampleofwhichis and (enzyme),thedirectrelatedresidueis and ,respectively.三．Makechoiceoneorseveral)：(3x8=SchiffbaseintermediatesareformedinthereactionscatalyzedA. B.glycogen C.Trp D.E.Multifunctional(orbifunctional)enzymeoccurspyrimidinesynthesisofeukaryotesb.FAsynthesisofeukaryotesC.thesynthesisandhydrolysisofF-2,6-BPD.thephosphorylationanddephosphorylationofisocitrateDHE.FAsynthesisofE.coliPRPPparticipatesinthesynthesisofthefollowingA.ProB.HisC.purineD.PheE.Biotin,asthepostheticgroupofthefollowingacetylCoAcarboxylaseB.transketolaseC.pyruvateD.PyrDHE.α–KGThefollowingcomplexcontainsFe-SA.NADH-QredueB.cytochromecredueC.cytochromecoxidaseD.succinate-QredueE.ribonucleotideredueWhichsubstanceisthedirectprecursorofthesynthesisofacetoacetate乙酰乙酸inliverHMGCoAB.acetoacetylCoAC.D-3-hydroxybutyrateD.D-3-hydroxybutyrylCoAE.Citricaciditcanproducehigh-energyphosphorylcompoundbyitselfB.itisnotinhibitedbytheanaerobic厭氧conditionC.thetwocarbonatomsofacetylCoAareliberatedastheformoftwoCO2inthatroundofthecycleD.itsratedependsupontheneedofATPE.itisthefinalcommonpathwayfortheoxidationoffuelmolecules.Itparticipatesintheactivatedmethylcycle(orS-AMetA.VB12 B.biotin C.FH4 D.VB2 Enicotinate煙酸四.Answerorcalculate:(7x3=21)WhatistheactivatedreactantineachoftheseSphingomyelin鞘磷脂fromceramideMetfromhomocysteinePhosphatidylcholinefromphosphatidylethanolamine乙IPPfrom5-pyrophosphomevalonateThesynthesisofphosphoribosylamineSerfromGlycogenn+1fromglycogenInpurinebiosynthesis,Asp eslinkedtothecarboxylategroupofanintermediate.Inthenextreaction,thecarbonskeletonoftheAspunitleavesintheformoffumurate延胡索酸.ThesereactionsarereminiscentofasimilarpairofreactionsinthedegradationofAA.Whichones?Currencyexchange.Foraproton-motiveforceof0.2V(matrixnegative),whatisthe Calculatethisratiothreetimes,assumingthatthenumberofprotonstranslocatedperATPformedis2,3,and4andthatthetemperatureis250C.2003TESTPAPER1)一hyperuricemiaLDLreceptorB相結(jié)合的細(xì)胞表面受體?？墒筁DL從血漿(發(fā)生細(xì)胞)LDL的合成從而調(diào)節(jié)血漿膽固醇的濃度。pyruvatedehydrogenasecomplex酸脫氫酶復(fù)合體?催化酸、NAD+和輔酶A之間A、NADHCO2(整個(gè)反應(yīng))的酶復(fù)合體。包含三個(gè)活性酶和五個(gè)輔酶。4.glucokinase(inliver)葡萄糖激酶哺乳動(dòng)物肝贓中特有的一種己糖激酶的同工酶,它與底物G-6-P的抑制，故可大量地吸納血液中(過(guò)多)的葡萄糖。malate-aspartateshuttle蘋果酸-(NADH中的電子)從從肝PRPP磷酸核糖焦磷生物合成多種含氮環(huán)狀化合物極其衍生物中必不可少的反應(yīng)物，特substratecycle:F-6-PF-1,6-P的互“macroCoA”巨”輔酶A”;指ACP(?；d體蛋白),它含有與輔酶A相同的磷酸泛酰巰基乙胺頭部(與脂肪酸合成過(guò)程中各中間物連接);77個(gè)氨ureacycle尿素循環(huán);一組循環(huán)反應(yīng),其中由氨基酸代謝產(chǎn)物兩個(gè)氨基和一個(gè)二氧化碳生UDP-glucose尿苷二磷酸葡萄糖;一種核苷二磷酸糖,作為糖原合成(和其它許多合成)2003TESTPAPER2)二B1,pyruvateDHcomplex,α-KGDHcomplex,酮脂酰-ACP還原酶,DH;羥脂酰-ACP脫水酶,延胡索酸酶;烯脂酰-ACP還原酶,DH高半胱氨酸甲基化形成甲硫氨酸,L-CoACoA,高半胱氨酸甲基轉(zhuǎn)移酶,CoA變位酶檸檬酸循環(huán)形成檸檬酸,糖異生形成葡萄糖,轉(zhuǎn)氨形成天冬氨酸,脫羧形成UDP-葡萄糖CDP-DAG,磷酸化,核苷三磷酸,MVA,3-HMGCoA,3-HMGCoA四氫葉酸,蝶啶,對(duì)氨基苯甲酸,谷氨酸S-腺苷甲硫氨酸,二氧化碳,10.G-6-PDH,LDL受體,內(nèi)因子,DH,NADH-Q生物素羧基載體