Hotline:400-820-3792Inhibitors ? ScreeningLibraries ? Proteinswww.MedChemEMoroxydineCat.No.:HY-W016937CASNo.:3731-59-7Synonyms:ABOB分子式:C?H??N?O分子量:171.2作用靶點(diǎn):InfluenzaVirus;HSV;HCV;Apoptosis;Caspase作用通路:Anti-infection;Apoptosis儲(chǔ)存方式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY生物活性Moroxydine(ABOB)是一種廣譜抗病毒化合物，對(duì)DNA和RNA病毒均具有多重抗病毒活性，包括流感病毒(influenzavirus)、單純皰疹病毒(herpessimplex)、水痘-帶狀皰疹病毒(varicellazoster)、麻疹病毒(measles)、腮腺炎病毒(mumpsdisease)、丙型肝炎病毒(hepatitisCvirus)等。Moroxydine(ABOB)對(duì)雙鏈RNA病毒和大DNA病毒感染的細(xì)胞展現(xiàn)出卓越的抗病毒效果且細(xì)胞毒性較低。Moroxydine(ABOB)在草魚腎臟細(xì)胞(CIK)中能夠阻斷草魚呼腸孤病毒(GCRV)誘導(dǎo)的細(xì)胞病變效應(yīng)，清除病毒核衣殼，維持細(xì)胞正常形態(tài)結(jié)構(gòu)。Moroxydine(ABOB)可顯著抑制細(xì)胞凋亡(apoptosis)過程，具體表現(xiàn)為：降低caspase-3酶活性、減少Bax蛋白表達(dá)，同時(shí)下調(diào)Bcl-2水平[1][2][3]。體外研究Moroxydinehydrochloride(HY-B0420A)usedinpapersMoroxydine(1-100μg/mL,24-96h)showsprotectiveeffectsagainstgrasscarpreovirus(GCRV)inCtenopharyngodonidellakidney(CIK)cells(TC50=1246.0μg/mL,SC=218.5μg/mL)andgrasscarpovary(GCO)cells(TC50=117.2μg/mL,SC=140.9μg/mL)[1][2].Moroxydine(1-100μg/mL,24-96h)showsprotectiveeffectsagainstgiantsalamanderiridovirus(GSIV)inepitheliomapapulosumcyprinid(EPC)cells,withaTC50of1486.3μg/mLandasafeconcentration(SC)of209.7μg/mL[2].Moroxydine(40μg/mL,48-96h)blocksthevirus-inducedCPEandcelldeathwithin96handmaintainedthenormalstructureandnucleocapsids[1].Moroxydine(40μg/mL,48-96h)significantlydecreasestheapoptosisinCIKcells(GCRV),GCOcells(GCRV)andEPCcells(GSIV)[1][2].Moroxydine(12-48h)significantlyinhibitsthecaspase3activity,Baxexpressionanddown-regulatesBcl-2at48hinCIKcells[1].1/3 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemEMoroxydine(20μg/mL,12-72h)showstime-dependentsuppressionofviralgeneexpressioninCIKcells(GCRV),GCOcells(GCRV)andEPCcells(GSIV)[1][2].Moroxydine(20μg/mL,72h)arrestscellcycleintheperiodofG1/SandG2/MofCIKcells[3].Moroxydine(3-7days)inhibitstheGCRV-inducedupregulationofimmunegenes(IL-1β,IFN-I,TNF-α)inthekidney,liver,muscleandgill[3].CellViabilityAssay[1]CellLine:GCRVinfectedCIKcellsConcentration:1,2.5,6.3,15.9,39.8,100μg/mLIncubationTime:24,48,72,96hResult:Presentedabetterprotective-efficiencythanRibavirin(HY-B0434).ShowednosignificantdifferencewhenexposeGCRV-infectedCIKcellsin39.8μg/mLcomparedwithvirus-freegroupat96h.ApoptosisAnalysis[1]CellLine:GCRVinfectedCIKcellsConcentration:40μg/mLIncubationTime:48hResult:ExhibitedapositiveinhibitionintheGCRVinfectedcellscomparedwithnegativecontrol.CellCycleAnalysis[3]CellLine:GCRVinfectedCIKcellsConcentration:20μg/mLIncubationTime:72hResult:ArrestedcellcycleintheperiodofG1/SandG2/MofCIKcellsRealTimeqPCR[1][3]CellLine:GCRVinfectedCIKcellsConcentration:20μg/mLIncubationTime:12,24,48,72hResult:Suppressedviralgenes(vp1,vp2,vp3andvp4)at12hfollowingaslowdecreasefrom12hto72hpostmedication.InhibitedtheIL-1βexpression.2/3 MasterofBioactiveMolecules—您身邊的抑制劑大師www.MedChemE客戶使用本產(chǎn)品發(fā)表的科研文獻(xiàn)? Pharmaceuticals(Basel).2024Sep19;17(9):1234.Seemorecustomervalidationsonwww.MedChemEREFERENCESYuXB,etal.MoroxydinehydrochlorideinhibitsgrasscarpreovirusreplicationandsuppressesapoptosisinCtenopharyngodonidellakidneycells.AntiviralRes.2016Jul;131:156-65.YuXB,etal.Invitroantiviralefficacyofmoroxydinehydrochlorideandribavirinagainstgrasscarpreovirusandgiantsalamanderiridovirus.DisAquatOrgan.2016Oct27;121(3):189-199.YuXB,etal.EffectsofmoroxydinehydrochlorideandribavirinonthecellulargrowthandimmuneresponsesbyinhibitionofGCRVproliferation.ResVetSci.2018Apr;117:37-44.McePdfHeightCauti