Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemESulforhodamineBsodiumsaltCat.No.:HY-D0974CASNo.:3520-42-1Synonyms:AcidRed52;KitonRed620分?式:C??H??N?NaO?S?分?量:580.65作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:50mg/mL(86.11mM;Needultrasonic)H2O:20mg/mL(34.44mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.7222mL8.6110mL17.2221mL5mM0.3444mL1.7222mL3.4444mL10mM0.1722mL0.8611mL1.7222mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)?內(nèi)使?。BIOLOGICALACTIVITY?物活性SulforhodamineBsodiumsalt?種熒光染料，可?于定量測定培養(yǎng)細(xì)胞的細(xì)胞蛋?量。體外研究SulforhodamineB(SRB)isoftenusedasamembrane-impermeablepolartracerorusedforcelldensitydeterminationviadeterminationofcellularproteins(cytotoxicityassay).TheSRBassayhasbeenusedto1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEinexpensivelyconductvariousscreeningassaystoinvestigatecytotoxicityincellbasedstudies.ThismethodreliesonthepropertyofSRB,whichbindsstoichiometricallytoproteinsundermildacidicconditionsandthencanbeextractedusingbasicconditions;thus,theamountofbounddyecanbeusedasaproxyforcellmass,whichcanthenbeextrapolatedtomeasurecellproliferation.Theprotocolcanbedividedintofourmainsteps:preparationoftreatment,incubationofcellswithtreatmentofchoice,cellfixationandSRBstaining,andabsorbancemeasurement.Thisassayislimitedtomanualorsemiautomaticscreening,andcanbeusedinanefficientandsensitivemannertotestchemotherapeuticdrugsorsmallmoleculesinadherentcells.Italsohasapplicationsinevaluatingtheeffectsofgeneexpressionmodulation(knockdown,geneexpressionupregulation),aswellastostudytheeffectsofmiRNAreplacementoncellproliferation[1].PROTOCOLCellAssay[1]Gentlyadd25μL(96-wellformat)or5μL(384-wellformat)cold50%(wt/vol)TCAtoeachwelldirectlytomediumsupernatant,andincubatetheplatesat4°Cfor1h.Mixingisnotrequired,asthiscouldleadtosomecellsdetachingfromthebottomofthewell.Washtheplatesfourtimesbysubmergingtheplateinatubwithslow-runningtapwaterandremoveexcesswaterbygentlytappingtheplateintoapapertowel.Afterthelastwashallowtheplatetoair-dryatroomtemperature.Add50μL(96-wellformat)or20μL(384-wellformat)of0.04%(wt/vol)SRBsolutiontoeachwell.Leaveatroomtemperaturefor1handthenquicklyrinsetheplatesfourtimeswith1%(vol/vol)aceticacid(200μLfor96-wellformator30μLfor384-wellformat)toremoveunbounddye.Allowtheplatetoair-dryatroomtemperature[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?AdvMater.2022May;34(21):e2200517.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].OrellanaEA,etal.SulforhodamineB(SRB)AssayinCellCulturetoInvestigateCellProliferation.BioProtoc.2016Nov5;6(21).pii:e1984.McePdfHeightCaution:Producthasnotbeenful