Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEEthidiumbromideCat.No.:HY-D0021CASNo.:1239-45-8Synonyms:EtBr;Homidiumbromide分?式:C??H??BrN?分?量:394.31作?靶點:Others作?通路:Others儲存?式:PleasestoretheproductundertherecommendedconditionsintheCOA.溶解性數(shù)據(jù)體外實驗H2O:≥100mg/mL(253.61mM)DMSO:83.33mg/mL(211.33mM;Needultrasonic)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.5361mL12.6804mL25.3608mL5mM0.5072mL2.5361mL5.0722mL10mM0.2536mL1.2680mL2.5361mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存?式和期限。BIOLOGICALACTIVITY?物活性Ethidiumbromide常?作分??物實驗學中的熒光標記(核酸染?)，如瓊脂糖凝膠電泳。體外研究Guidelines(Followingisourrecommendedprotocol.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds).Thepreparationofagarosegel:1．Agarosegelsarecommonlyusedinconcentrationsof0.5%to2.5%dependingonthesizeofbands1/2MasterofSmallMolecules—您?邊的抑制劑?師www.MedChemEneededtobeseparated.2．Mixtheagarosepowderwith1XTAE/TBE.3．Microwavefor1-3minuntiltheagaroseiscompletelydissolved(Caution:notoverboil).4．Makethesolutioncooldownbeforesolidification.5．Addethidiumbromide(EtBr)toafinalconcentrationofapproximately0.2-0.5μg/mL(Stocksaregenerally10mg/ml,andrequire5μLstock/100mLgel).6．EthidiumbromidebindstotheDNAandyoucouldvisualizetheDNAunderultraviolet(UV)light.CAUTION:EtBrisaknownmutagen.Pleasepayattentiontostrengtheningprotection.REFERENCES[1].PStevenson,etal.ComparisonofisometamidiumchlorideandhomidiumbromideasprophylacticdrugsfortrypanosomiasisincattleatNguruman,Kenya.ActaTrop.1995May;59(2):77-84.[2].Sabnis,RamWasudeo(2010).HandbookofBiologicalDyesandStains:SynthesisandIndustrialApplication.Hoboken,NJ:Wiley.ISBN978-0-470-40753-0.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:4